BIOTA Philippines Conference System, 52nd BIOTA Annual National Convention and Scientific Sessions

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DNA BARCODING AND GENETIC DIVERSITY OF IXORA SPP. COLLECTED IN GUIMARAS ISLAND, PHILIPPINES
Rizza _ Carreon

Last modified: 2017-03-13

Abstract


Ixora is considered to be one of the largest genera in Rubiaceae of trees and shrubs and is characterized by having striking inflorescences and tetramerous flowers. To augment the morphological identification of this taxon, three candidate chloroplast DNA barcodes (matK, rbcL, and trnL-F) were evaluated for their efficiency to molecularly authenticate Ixora species. Several population of Ixora spp. (I. finlaysoniana and I. philippinensis) were collected in Guimaras Island, Visayas, Philippines. Genomic DNA was extracted from silica-dried leaf samples. Specific DNA region (matK, rbcL, and trnL-F) were amplified, purified and sequenced. Newly generated sequences were edited, aligned and evaluated based on the three criteria of DNA barcoding; universality, sequence quality and discriminatory power using the MEGA software. For sequence quality, trnL-F provided the shortest contig with the highest number of informative characters. Meanwhile for universality, rbcL and trnL-F both yielded the highest percentage in PCR amplification and sequencing success rate. As for the discriminatory power, pairwise sequence divergence revealed that matK provided the highest interspecific mean divergence while rbcL showed the lowest intraspecific mean divergence.  The genetic diversity of the species of Ixora was also determined and results showed that trnL-F provided the highest haplotype number while rbcL provided the lowest haplotype number.


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