BIOTA Philippines Conference System, 52nd BIOTA Annual National Convention and Scientific Sessions

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Utilization of Soursop (Annona muricata) Peeling as Culture Media for Fungi
Realyn Carbilla De Jesus, Joshua Tomo Soriano, Jan Michaella Dalanon Geromiano, John Ernest Ganancios orinas, Marvin Rey Arca Casamena

Last modified: 2017-03-13

Abstract


Soursop peeling is mainly composed of carbohydrate which is commonly disposed by people. As commercially available culture media are expensive, there is a need to find alternative sources to lower the cost involved.This study examined the potential of soursop (Annona muricata) peeling as an alternative starch source in the formulation of fungal cultivation medium. Test organisms used were Pleurotus florida, Ganoderma lucidum and Pleurotus sajorcaju. Soursop Dextrose Agar (SDA )was formulated based on its carbohydrate content and consisted of 20g dextrose, 20g agar, and 250 g of soursop peelings. Area covered by fungal mycelia was compared with the conventional Potato Dextrose Agar (PDA). Results showed a comparable fungal growth between SDA and PDA using the three fungal species. Pleurotus florida cultured in SDA had an average growth rate of 4.60 cm2 while those cultured in PDA had 5.50 cm2. Ganoderma lucidum cultured in SDA exhibited an average growth rate of 5.50 cm2 while those cultured in had PDA 8.60 cm2 and Pleurotus sajurcaju cultured in SDA had an average growth rate 13.00 cm2 while those cultured in PDA had 10.00 cm2. This study indicates that soursop peeling is a good alternative source for fungal culture medium and can be used in mycological studies.

Keywords: PDA, SDA, potential starch source, soursop peeling , culture media

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