BIOTA Philippines Conference System, 52nd BIOTA Annual National Convention and Scientific Sessions

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Evaluating the Developmental Instability of Clupea sp. from Surigao City, Philippines Using Fluctuating Asymmetry
Hannah Nier Abarca

Last modified: 2017-03-13


Landmark- based geometric morphometric analysis is a more sophisticated tool to describe and quantify the asymmetry of biological shapes. The level of fluctuating asymmetry of Clupea sp. was used to assess the condition of fishing waters surrounding the premise of Surigao City. A total of 60 male and female individuals were digitized and landmarked using Thin – Plate Spline (TPS) for landmark analysis of each sample and were subjected to Symmetry and Asymmetry Data (SAGE) software. Results on Procrustes ANOVA revealed highly significant differences in the three factors considered (P<0.005) for males. These factors were individual, sides and interaction of individual and sides. Results showing high variations (P<0.005) on the left and right side of the bilateral with a percentage of 87.4834% in male and 92.82% in female indicates high Fluctuating Asymmetry. Similar FA results were observed to both male and female samples except for one factor which is the individual symmetry for females. The affected landmarks were further analyzed using principal component analysis (PCA). PCA results showed all landmarks are affected. PCA also showed skewness in the deformation grid which also signifies FA. This study validated the use of FA in evaluating the water quality and environmental health of Surigao City fishing premise.

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