BIOTA Philippines Conference System, 52nd BIOTA Annual National Convention and Scientific Sessions

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STUDY OF THE EMBRYO-TOXIC AND TERATOGENIC EFFECTS OF Brassica junicea SEED EXTRACT IN ZEBRAFISH EMBRYOS
Ephraim Martinez Villacrusis

Last modified: 2017-03-13

Abstract


In the present study, the embryo-toxic and teratogenic activities of the Brassica junicea seed ethanolic crude extract were examined in zebrafish embryo model. Experiments were conducted by preparing four formulations of the crude extracts with 100%, 50%, 25%, and 12.5 % B. junicea seed crude extract. Positive and negative controls were established by using the embryo medium as the positive control and 2% ethanol as the negative control. Treatments are applied to the six replicates. Observation in the third (3) and fifth (5) day post-fertilization (dpf) period was made focusing on the mortality, hachability, heart beat rate, mean length, number of melanocytes  and abnormalities.

 

Results revealed that the Brassica junicea seed extracts were not embryo-toxic with different concentrations. Hatching rate at 3 dpf were found dependent on dose and time of exposure. Among the four extracts, 100%, 50%, and 25% concentrations of seed extracts show 100%, 80% and 81% retardation on the hatching rate, respectively. While, 12.5% concentrations show a 100 % hatching rate. It is also recorded that embryos exposed at 100%, 50% and 25% concentrations of seed extracts exhibit decrease in heart beat rate at 140.8 ,172, and 174.9 beats per minute, respectively. The highest mean length of 3.22 mm was recorded at 50 % concentration of seed extracts. Number of melanocytes of zebrafish at 5 dpf was not affected by the varying concentration of the seed extracts. The teratogenic effect of Brassica junicea in zebrafish embryo at 5 dpf reveals significant difference between different treatments although it is limited with the delay on the hatching rate.  Despite  the various bioactivities of Brassica junicea, seed extracts of these plants also exhibit teratogenic effects in the developing embryos of zebrafish.

 

Keywords: Teratogenicity, Brassica junicea, Embryo-toxic and Zebrafish model


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