BIOTA Philippines Conference System, 52nd BIOTA Annual National Convention and Scientific Sessions

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INSTRUMENTAL ANALYSIS, PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ANALYSID OF MOLLUGO OPPOSITIFOLIA (PAPAIT)
Lorna T. Enerva

Last modified: 2017-03-10

Abstract


1,2Lorna T. Enerva, 1Mary Angelica Arevalo, 1Jimboy M. Cayetano,1Marcon T. Co Jr et al

1College of Science, Polytechnic University of the Philippines

2College of Education, Taguig City University

E-mail: lenyenerva@yahoo.com

In this study the researchers made a partial characterization, phytochemical screening and antimicrobial determination of Mollugo oppositifolia.Mollugo oppositifolia. is also known as Papait, Malagoso Sarsalida and Amargoso. It is a slender, spreading or ascending herb with branches as long as 10 to 40 centimeters. Leaves are opposite or whorled, spatulate, oblanceolate to oblong-ovate, 1 to 3 centimeters long, and up to one centimetre wide. In folklore literature, papait is used as stomachic and as uterine stimulant. The whole plant is used as cataplasm for dyspepsia in children.

The leaves were gathered from Guimba, Nueva Ecija and soaked in ethyl acetate for two weeks, purified by column chromatography using n-hexane-ethyl acetate and ethanol as solvents.

Phytochemical screening showed that the plant extract contains amino acids, fixed fats and oils, saponins and tannins. Infrared spectra showed the presence aliphatic hydrocarbons, aldehydes C=O at 1741.04 cm-, aromatic ring at 1633.22 cm- and C=C at 1452.12 cm-. The UV absorption was at λmax = 228.0 nm meaning that the compound contains  auxochromes and chromophoric groups.

The plant extract inhibited the growth of S. aureus and C.albicans with AI of 1.7 and 2.2 respectively. The AI of the plant extract and the control canesten to C. albicans are the same 2.2 AI implying that this plant had a very high antifungal property. No zone of inhibition was observed in the plate of P. aeruginosa.

 

 


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